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SNPAssayR 1.0.1
- Initial release: offline CAPS, single-substitution site-creation
dCAPS, conventional two-reaction ARMS and tetra-primer ARMS candidate
design.
- Evaluate perfect-match primer thermodynamics, sequence compatibility
and theoretical complete digests with explicit uncertainty and search
limits.
- Provide source-linked coordinate and band schematics, within-method
preference ranking, reproducible exports and an optional local Shiny
interface.
- Apply tetra-primer product-size bounds to the control and both
diagnostic products; retain separate broad pairwise risk screening.
- Include runnable public-API examples and regenerable computational
references.
- Expand the source-verified enzyme panel to 20 distinct motifs,
including MfeI-HF.
- Add an offline 550-bp synthetic teaching locus and practical-length
workflows for CAPS, dCAPS, conventional ARMS and tetra-primer ARMS.
- Provide a version-specific software citation through
citation(“SNPAssayR”).
- Record ICAR-Indian Institute of Rapeseed-Mustard Research
(ICAR-IIRMR) as copyright holder while retaining the three authors and
designated maintainer.
These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.