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Loading the example data

pGRN package pre-loaded with pGRNDB data with both of expression matrix and pseudotime information. You can simply load this data by calling pGRNDB.

# loading pGRN package
library(pGRN)

# loading pre-build data
example_data <- pGRNDB
names(example_data)

## [1] "expression" "ptime"

expression_matrix <- example_data[["expression"]]
pseudotime_list <- example_data[["ptime"]]$PseudoTime

Call pGRN

Currently, two methods “DTW” and “granger” were available for pGRN network construction.

# try DTW method
nets_dtw <- pGRN(expression_matrix,pseudotime_list, method= "DTW",quantile_cutoff=50)

# try granger method
nets_gg <- pGRN(expression_matrix,pseudotime_list, method= "granger")

## Time relapsed for granger test: 1.6192889213562 seconds!

plot the network

pGRN provides methods for visualize the network either stationary or interactively.

# plot network stationarily
plot_network(nets_dtw[[1]])

plot_network(nets_gg[[1]])

# plot network interactively
#plot_network_i(nets_dtw[[1]])
#plot_network_i(nets_gg[[1]])

These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.