FracFixR: Compositional Statistical Framework for RNA Fractionation Analysis

A compositional statistical framework for absolute proportion estimation between fractions in RNA sequencing data. 'FracFixR' addresses the fundamental challenge in fractionated RNA-seq experiments where library preparation and sequencing depth obscure the original proportions of RNA fractions. It reconstructs original fraction proportions using non-negative linear regression, estimates the "lost" unrecoverable fraction, corrects individual transcript frequencies, and performs differential proportion testing between conditions. Supports any RNA fractionation protocol including polysome profiling, sub-cellular localization, and RNA-protein complex isolation.

Version:	1.0.0
Depends:	R (≥ 4.0.0)
Imports:	future.apply (≥ 1.8.1), nnls (≥ 1.4), ggplot2 (≥ 3.3.5), dplyr (≥ 1.0.7), RColorBrewer (≥ 1.1-2), tidyr (≥ 1.1.3), matrixStats (≥ 0.60.0), aod (≥ 1.3.1), stats, utils, future, grDevices
Suggests:	testthat (≥ 3.0.0), knitr, rmarkdown
Published:	2025-10-21
DOI:	10.32614/CRAN.package.FracFixR (may not be active yet)
Author:	Alice Cleynen [aut, cre], Agin Ravindran [aut], Nikolay Shirokikh [aut]
Maintainer:	Alice Cleynen <alice.cleynen at cnrs.fr>
License:	CC BY 4.0
NeedsCompilation:	no
Materials:	NEWS
CRAN checks:	FracFixR results

Documentation:

Reference manual:	FracFixR.html , FracFixR.pdf
Vignettes:	Introduction to FracFixR (source, R code)

Downloads:

Package source:	FracFixR_1.0.0.tar.gz
Windows binaries:	r-devel: not available, r-release: not available, r-oldrel: not available
macOS binaries:	r-release (arm64): FracFixR_1.0.0.tgz, r-oldrel (arm64): FracFixR_1.0.0.tgz, r-release (x86_64): FracFixR_1.0.0.tgz, r-oldrel (x86_64): FracFixR_1.0.0.tgz

Linking:

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