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rtpcr: qPCR Data Analysis

Tools for qPCR data analysis using Delta Ct and Delta Delta Ct methods, including t-tests, ANOVA, ANCOVA, repeated-measures models, and publication-ready visualizations. The package supports multiple target, and multiple reference genes, and uses a calculation framework adopted from Ganger et al. (2017) <doi:10.1186/s12859-017-1949-5> and Taylor et al. (2019) <doi:10.1016/j.tibtech.2018.12.002>, covering both the Livak and Pfaffl methods.

Version: 2.1.1
Depends: R (≥ 3.5.0)
Imports: multcomp, ggplot2, lmerTest, purrr, reshape2, tidyr, dplyr, grid, emmeans
Suggests: knitr, rmarkdown, multcompView
Published: 2026-01-08
DOI: 10.32614/CRAN.package.rtpcr
Author: Ghader Mirzaghaderi [aut, cre, cph]
Maintainer: Ghader Mirzaghaderi <gh.mirzaghaderi at uok.ac.ir>
License: GPL-3
URL: https://mirzaghaderi.github.io/rtpcr/, https://github.com/mirzaghaderi/rtpcr
NeedsCompilation: no
Citation: rtpcr citation info
Materials: NEWS
CRAN checks: rtpcr results

Documentation:

Reference manual: rtpcr.html , rtpcr.pdf
Vignettes: Manual (source, R code)
Calculation method (source, R code)

Downloads:

Package source: rtpcr_2.1.1.tar.gz
Windows binaries: r-devel: rtpcr_2.1.1.zip, r-release: rtpcr_2.1.1.zip, r-oldrel: rtpcr_2.1.1.zip
macOS binaries: r-release (arm64): rtpcr_2.1.1.tgz, r-oldrel (arm64): rtpcr_2.1.1.tgz, r-release (x86_64): rtpcr_2.1.1.tgz, r-oldrel (x86_64): rtpcr_2.1.1.tgz
Old sources: rtpcr archive

Linking:

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These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.