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freqpcr: Estimates Allele Frequency on qPCR DeltaDeltaCq from Bulk Samples

Interval estimation of the population allele frequency from qPCR analysis based on the restriction enzyme digestion (RED)-DeltaDeltaCq method (Osakabe et al. 2017, <doi:10.1016/j.pestbp.2017.04.003>), as well as general DeltaDeltaCq analysis. Compatible with the Cq measurement of DNA extracted from multiple individuals at once, so called "group-testing", this model assumes that the quantity of DNA extracted from an individual organism follows a gamma distribution. Therefore, the point estimate is robust regarding the uncertainty of the DNA yield.

Version: 0.4.0
Depends: R (≥ 3.6), cubature
Imports: methods
Suggests: knitr, rmarkdown, remotes
Published: 2022-01-27
DOI: 10.32614/CRAN.package.freqpcr
Author: Masaaki Sudo ORCID iD [aut, cre]
Maintainer: Masaaki Sudo <masaaki at sudori.info>
License: GPL (≥ 3)
URL: https://github.com/sudoms/freqpcr
NeedsCompilation: no
Citation: freqpcr citation info
Materials: README NEWS
CRAN checks: freqpcr results

Documentation:

Reference manual: freqpcr.pdf
Vignettes: Introducing freqpcr

Downloads:

Package source: freqpcr_0.4.0.tar.gz
Windows binaries: r-devel: freqpcr_0.4.0.zip, r-release: freqpcr_0.4.0.zip, r-oldrel: freqpcr_0.4.0.zip
macOS binaries: r-release (arm64): freqpcr_0.4.0.tgz, r-oldrel (arm64): freqpcr_0.4.0.tgz, r-release (x86_64): freqpcr_0.4.0.tgz, r-oldrel (x86_64): freqpcr_0.4.0.tgz

Linking:

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These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.